Journal: Frontiers in Pharmacology
Article Title: Modified Si Miao Powder granules alleviates osteoarthritis progression by regulating M1/M2 polarization of macrophage through NF-κB signaling pathway
doi: 10.3389/fphar.2024.1361561
Figure Lengend Snippet: MSMP was involved in immunoregulation of synovial macrophages. (A) Immunohistochemical staining of Arg1. (B) Statistical analysis of the positive expression for Arg1 in synovial tissue. (C) Immunohistochemical staining of iNOS. (D) Statistical analysis of the positive expression for iNOS in synovial tissue. Scale bar represents 100 μm (Date are mean ± SD, #### p < 0.0001, versus Sham group; ** p < 0.01, *** p < 0.001, **** p < 0.0001, versus OA group).
Article Snippet: The reagents in the current study were as follows: Lipopolysaccharide (Sigma), p65 Antibody (Abways, CY5034), p-p65 Antibody (Abways, CY6372), β-actin Antibody (Boster, BM3873), Col2a1 Antibody (Boster, BA0533), Mmp13 Antibody (Boster, BA2204), iNOS Antibody (Boster, BA0362), Arg1 Antibody (Servicebio, GB11285-100), IL-6 ELISA Kit (Boster), TNF-α ELISA Kit (Boster), IL-10 ELISA Kit (RuiXin Bio), HE Staining Kit (Solarbio), Senna O Solid Green Staining Kit (Solarbio) Animal Surgical Instruments, Absorbable Surgical Suture (6-0), Gavage Needle (12 gauge), Tissue Decalcification Solution (Bosterbio), Tissue Fixation Solution (Bosterbio), and Sodium Pentobarbital.
Techniques: Immunohistochemical staining, Staining, Expressing